EFFECT OF DIFFERENT CYTOKININ CONCENTRATIONS ON IN VITRO SHOOT MULTIPLICATION OF BAMBUSA TULDA ROXB. FROM NODAL CUTTINGS

Abstract

Bambusa tulda Roxb. is an economically important bamboo species that belongs to the order Poales, family Poaceae and sub-family Bambusoideae. It is a sympodial species that grows well in tropical and sub-tropical regions of India. Bambusa tulda is tall, sturdy and quickly growing bamboo species having succulent shoots that are rich in phytosterols. The species is overexploited due to its economic importance. Large scale production is not sufficient by the conventional method of propagation due to their low multiplication rate, time consuming, high cost and labor intensive. For the conservation and mass propagation of different bamboo species, in vitro culture is providing to be a promising process. So, in the present study, an experiment was conducted to achieve a protocol for an effective concentration of cytokinin on the shoot proliferation and multiplication of Bambusa tulda Roxb. using nodal cuttings. The nodal explants were used to induce multiple shoots proliferation using various concentration of BAP and Kn on MS medium. The MS medium supplemented with BAP (3.0mg/l) supported the maximum in vitro shoot multiplication. The result of the experiment also showed that, 3 mg/l BAP concentration was found to be more effective for shoot proliferation of Bambusa tulda.