A NOVEL D-ALANINE SENSOR USING SURFACE PLASMON RESONANCE PROPERTY OF SILVER NANO PARTICLES FOR MICRO DETECTION IN SIMULATED BODY FLUID

Abstract

High D-alanine concentration is implicated in Alzheimer’s disease and blood plasma of patients with renal disorders. A novel, simple D-alanine sensor was developed using the localized surface plasmon resonance property of silver nanoparticles (SNP). The amount of decrease in surface plasmon resonance band of SNP due to H₂O₂ produced during the D-amino acid oxidase reaction corresponded to the concentration of D-alanine which could be directly monitored using the spectrophotpmetric methods. The sensor was used for the detection of D-alanine in human blood plasma mimicking fluid i.e. simulated body fluid which showed linear detection in the range of 0.1mM to 5mM D-alanine concentration.